产品货号：
GS2152
中文名称：
Ple19I内切酶
英文名称：
Ple19I
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：Ple19I内切酶，Ple19I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(HindIII-digest)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | CGAT↑CG |
| 来源 | Pseudomonas lemoignei 19 |
| Assayed on | Lambda DNA(HindIII-digest) |
| Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);100 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 3-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Not blocked by overlapping Dam-methylation(GmATC):CGATCG |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BpvUI,BspCI,MvrI,PvuI |
相关搜索：Ple19I内切酶，Ple19I限制性内切酶